JAK3i

Protein target name: 
JAK3
Mechanism of action: 
Irreversible inhibitor

Target Details

Target class: 
Protein kinase
Subclass: 
Tyrosine kinase
Target alias: 
JAK-3_HUMAN, JAKL, L-JAK, LJAK, JAK3, Janus kinase 3

Probe Details

Chemical Probes Portal ID: 
CPP657
SMILES string: 
CCOC(=O)C1=CNC2=NC=NC(=C12)C3=C(C=CC(=C3)NC(=O)C=C)F
InChi Key: 
KNLROUBMVYVLGX-UHFFFAOYSA-N
Control compound: 
Compound 2 from Smith et al. 2016 (propionamide of JAK3i)
Orthogonal probe: 
Compound 3 from Goedken et al. JBC 290 4573-4589 (2015)Compound 45 from Tan et al. J. Med. Chem. 58 6589-6606 (2015)

Physico-chemical properties

Solubility - preferred solvent: 
DMSO
Solubility - concentration: 
>10 mM
NMR: 

1H NMR (400 MHz, DMSO): 13.04 (br s, 1H), 10.33 (s, 1H), 8.97 (s, 1H), 8.33 (s, 1H), 8.06 (dd, J = 2.7, 6.6 Hz, 1H), 7.83 (ddd, J = 2.7, 4.7, 9.0 Hz, 1H), 7.23 (dd, J = 9.0, 9.0 Hz, 1H), 6.44 (dd, J = 10.2, 16.8 Hz, 1H), 6.28 (dd, J = 2.0, 16.8 Hz, 1H), 5.77 (dd, J = 2.0, 10.2 Hz, 1H), 4.01 (br s, 1H), 3.88 (br s, 1H), 0.94 (t, J = 7.4 Hz, 3H).

13C NMR (100 MHz, DMSO): 163.1, 162.8, 156.1 (J = 243 Hz), 153.6, 153.0, 151.6, 135.0 (J = 2.3 Hz), 134.6, 131.7, 127.5 (J = 15.3 Hz), 127.0, 121.8 (J = 8.4 Hz), 121.5 (J = 3.1 Hz), 115.2 (J = 23.7 Hz), 113.8, 107.7 (J = 3.1 Hz), 59.5, 13.7. ESI-MS: 355.4 (M+H+).

Storage Recommendations
Storage (dry powder): 
Room temperature
Storage (solution): 
-20 C, stable for >2 years.
Storage sensitivities: 
Not sensitive to freeze-thaw.

Primary Reference