EPZ015666

Reagent Authentication Certificate: 
PDF icon Chemical Probes Portal EPZ015666.pdf
Protein target name: 
PRMT5
Mechanism of action: 
Protein substrate competitive inhibitorSAM uncompetitive inhibitor

Target Details

Target class: 
Epigenetics
Subclass: 
Protein methyltransferase
Target alias: 
JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5

Probe Details

Chemical Probes Portal ID: 
CPP154
Probe alias: 
1616391-65-1, 3XV, GTPL8287
SMILES string: 
C1CN(CC2=CC=CC=C21)C[C@H](CNC(=O)C3=CC(=NC=N3)NC4COC4)O
InChi Key: 
ZKXZLIFRWWKZRY-KRWDZBQOSA-N
Control compound: 
EPZ019896
PAINs evaluation: 
PAINs free

Physico-chemical properties

NMR: 

1H NMR (400MHz, MeOD-d4) δ 8.24 (s, 1H), 7.15 - 7.05 (m, 4H), 7.02 - 6.98 (m, 1H), 5.09 (s, 1H), 4.95 (t, J=6.8 Hz, 2H), 4.59 (t, J=6.3 Hz, 2H), 4.10 - 4.03 (m, 1H), 3.72 (s, 2H), 3.56 - 3.46 (m, 2H), 2.96 - 2.91 (m, 2H), 2.87 - 2.80 (m, 2H), 2.65 (d, J=6.3 Hz, 2H);

13C NMR (101 MHz, DMSO-d6) δ ppm 29.7, 45.7, 47.4, 52.8, 57.6, 63.7, 68.1, 79.4, 104.9, 126.8, 127.4, 127.6, 129.6, 135.2, 135.5, 155. 8, 158.8, 164.1, 165.9

Storage Recommendations
Storage (solution): 
-20 oC for 2 years

Primary Reference