1G244

Protein target name:

DPP8, DPP9

Mechanism of action:

Inhibitor

Recommended Concentration for use in cells:

up to 8 uM

Probe Availability

MedChemExpress

Probe Information

Target and Probe Information

Target Details

Target class:

DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141; DP9; DPLP9; DPRP2; DPP IX; DPRP-2

Probe Details

Chemical Probes Portal ID:

CPP2300

Probe alias:

9XH, cpd 1

PubChem CID:

56658139

ChEMBL ID:

CHEMBL1814633

SMILES string:

N[C@@H](CC(=O)N1CCN(C(c2ccc(F)cc2)c2ccc(F)cc2)CC1)C(=O)N1Cc2ccccc2C1

InChi Key:

ZKIQFLSGMMYCGS-SANMLTNESA-N

Validation in Cells and Model Organisms

In vitro validation

On target activity

Potency:

IC50, Ki

14 nM DPP8, 53 nM DPP9 (IC50)0.9 nM DPP8, 4.2 nM DPP9 (Ki)

Potency assay:

Enzymatic assay

PDB ID for probe-target interaction (3D structure):

6TRX, 6EOR

Off target activity

Probe Selectivity in Vitro:

Selective over: DPP4 >100,000, FAP >100,000, DPP2 >100,000

In cell validation

On target activity in cells

Potency in cells:

IC50

2.7 uM

Potency assay (cells):

Cell viability (CellTiter-Glo) assay

Toxicity

Cytoxicity assay:

Yes

Notes on cytotoxicity:

1G244 shows minimal cytotoxicity against several mammalian cancer cell lines, with CC50 (cytotoxic concentration) values well over 20 μM.

In vivo validation

Organism:

Rat

Dose:

1, 10, 30 mg/Kg1.1 (IV), 2.2 (PO) mg/Kg

Route of delivery:

OralIntravenous

Plasma half life:

7 (IV), 1 (PO) h

Systemic clearance:

23.4 (IV) ml/min/Kg

Cmax:

0.2 nmol/ml (PO)

Vss:

3.2 (IV) L/Kg

AUC:

1.6 (IV), 0.5 (PO) nmol/(mL*h)

Bioavailability:

16.5 % (PO)

Primary Reference

Reference (doi):

10.1039/C5MD00454C 10.1016/j.bcp.2009.03.032 10.1038/nchembio.2229

Reference (PMID):

19439267 27820798

1G244

Probe Availability

Probe Information

Target Details

DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141; DP9; DPLP9; DPRP2; DPP IX; DPRP-2

Probe Details

In vitro validation

In cell validation

Toxicity

In vivo validation

Primary Reference

Supporting Organizations

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1G244

Probe Availability

Probe Information

Target Details

DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141; DP9; DPLP9; DPRP2; DPP IX; DPRP-2

Probe Details

In vitro validation

In cell validation

Toxicity

In vivo validation

Primary Reference

Supporting Organizations

Interested in supporting the portal?