Potency assay (cells):
Stimulation of insulin secretion in rat INS-1E cells after 1 hr by alphaLISA assay in presence of 5 mM glucose (Bioorg. Med. Chem. Lett., (2014) 24:4:1031). In HIT (hamster beta cell line) cells, glyburide stimulates insulin secretion with ED50=0.4 nM.
Target engagement assay (cells):
In HIT cells, an iodinated version of glyburide photolabeled ABCC8; this labeling was blocked by unmodified glyburide.