FT671

FT671 : Inhibitor of USP7

Structure

SERP Rating

In Cells

(2 ratings)

In Model Organisms

(0 ratings)

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Probe FT671 is in the process of SERP review.

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Information

USP7

Inhibitor

up to 1 uM
Reviewer recommended concentration: 1 to 10 µM

In Vitro Validations

Uniprot ID: Q93009

Target Class: Enzyme

Target SubClass: Peptidase

Potency: Kd

Potency Value: 65 nM

Potency Assay: SPR

PDB ID for probe-target interaction (3D structure): 5NGE

Target aliases:

Ubiquitin carboxyl-terminal hydrolase 7, HAUSP, US ...

DOI Reference: 10.1038/nature24451

Uniprot ID: Q93009

Target Class: Enzyme

Target SubClass: Peptidase

Potency: IC50

Potency Value: 52 nM

Potency Assay: In vitro USP7 activity assay (FRET)

PDB ID for probe-target interaction (3D structure): 5NGE

Target aliases:

Ubiquitin carboxyl-terminal hydrolase 7, HAUSP, US ...

DOI Reference: 10.1038/nature24451

In Cell Validations

In Vivo Data

No in Vivo Validations

Off-Target Selectivity Assesments

Potency assay (off target): FT671 xclusively inhibit USP7 in a panel of 38 deubiquitinases (DUBs) from diverse families at 50 uM. FT671 was also tested against USP47 and USP10 with no effects on their activity.

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SERP ratings and comments

SERP Ratings

In Cell Rating

SERP Comments:

Well-characterized, selective inhibitor of USP7 suitable for use in cellular experiments. The original publication shows an effect in a mouse xenograft model derived from a cell line that is sensitive to ST671 (as shown in in-cell validation 1).

(last updated: 29 Mar 2024 )

SERP Ratings

In Cell Rating

SERP Comments:

The quality of the probe should be tested after long-term storage ensuring it is not degraded, still pure etc

(last updated: 9 Apr 2024 )