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ABPA3 | ABPA3 : Covalent Inhibitor of UBA1, UBA3
RATINGS:
Cellular Use: (3 reviews)

In Model Organisms: (2 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
UBA1
  • IC 50:43 nM
  • IC 50:2500 nM
UBA3
  • IC 50:43 nM
  • IC 50:80 nM
Covalent Inhibitor
3-5 uM for 16h

Selectivity

In Vitro Selectivity Assessment
Selectivity Assessment Description:
ABP A3 is an AMP mimic and therefore can inhibit protein kinases. Its close analogue MLN4924 was pro ...
In Cell Selectivity Assessment
Potency Assay Off-Target:
Cells were treated with ABP A3 and covalent ABP A3-Ubl adducts were visualized using in gel scanning ...
Selectivity Assessment Description:
We also evaluated if ABP A3 inhibits closely related analogues SUMO E1, ISG15 E1, Ufm1 E1. We did no ...

Potency
Cellular
In Vitro

UBA1

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1039/C5SC01351H

UBA3

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1039/C5SC01351H

Negative Control Compounds

N/A

Orthogonal Probes def

MLN7243
TAK-243
Pevonedistat

Chemical Information

Molecular Formula C18H18N6O6S
SMILEs C#Cc1cccc(Nc2ncnc3c2ncn3[C@@H]2O[C@H](COS(N)(=O)=O)[C@@H](O)[C@H]2O)c1
InChI InChI=1S/C18H18N6O6S/c1-2-10-4-3-5-11(6-10)23-16-13-17(21-8-20-16)24(9-22-13)18-15(26)14(25)12(30-18)7-29-31(19,27)28/h1,3-6,8-9,12,14-15,18,25-26H,7H2,(H2,19,27,28)(H,20,21,23)/t12-,14-,15-,18-/m1/s1
Molecular weight 446.10 Da
AlogP -0.6094999999999993
HBond acceptors 12
HBond donors 5
Atoms 49

Expert Reviews

by SERP
(on 6 Sept 2016)
Cellular Use Rating
ABPA3 is an activity-based probe that covalently inhibits the ubiquitin- and Nedd8-activating E1 enzymes (UBA1/UBA3), resulting in inhibition of both Nedd8-dependent and Nedd8-independent ubiquitin...
by SERP
(on 27 Dec 2016)
Cellular Use Rating
In Model Organisms
The published characterization of ABPA3 rests largely on phenotypic, cell-based proteomics in A549 cells. The results, showing selective formation of covalent complexes between ABPA3 and ubiquitin and...
by SERP
(on 13 Jul 2017)
Cellular Use Rating
In Model Organisms
This compound is a non-specific, covalent inhibitor of ubiquitinylation and Nedd8 and should therefore be used with caution as a single agent, as this polypharmacolgy will almost certainly confound...
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