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EED226 | EED226 : Allosteric inhibitor of EED
RATINGS:
Cellular Use: (4 reviews)

In Model Organisms: (3 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
EED
  • Kd:82 nM
  • IC50:23.4 nM
Allosteric
100 nM - 1 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
EED226 had limited activity against 21 protein methyltransferases (IC50 >100 uM). EED226 inhibited ...
Selectivity Assessment Description:
EED226 had limited activity against 23 kinases (IC50 >10 uM), and 22 GPCRs, ion channels, nucl ...

Potency
Cellular
In Vitro

EED

Mode of Action: Allosteric

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1038/nchembio.2304

In Vivo Validations

Mouse
Dose: 4 and 40 mg/kg
Route of delivery: Oral
Organ of interest (O): Karpas422 xenograft
Target engagement assay: EED226 led to dose-dependent decrease in global H3K27me3 levels.

DOI Reference: 10.1038/nchembio.2304

Orthogonal Probes def

A-395

Chemical Information

Molecular Formula C17H15N5O3S
SMILEs CS(=O)(=O)c1ccc(-c2cnc(NCc3ccco3)n3cnnc23)cc1
InChI InChI=1S/C17H15N5O3S/c1-26(23,24)14-6-4-12(5-7-14)15-10-19-17(22-11-20-21-16(15)22)18-9-13-3-2-8-25-13/h2-8,10-11H,9H2,1H3,(H,18,19)
Molecular weight 369.09 Da
AlogP 2.3999000000000006
HBond acceptors 8
HBond donors 1
Atoms 41

References

Publications

Cross References

Vendors

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Expert Reviews

by SERP
(on 13 Jun 2017)
Cellular Use Rating
The fact that this compound is non-competitive with respect to SAM and peptide substrate is supported by an X-ray structure and enzymatic competition assays. The furane ring with electron-donating group...
by SERP
(on 14 Jun 2017)
Cellular Use Rating
In Model Organisms
This allosteric inhibitor of PRC2 competes with the H3K27me3 peptide for binding to EED. So far, it seems to be a very clean inhibitor. The only data on this compound has been published by a single...
by SERP
(on 16 Jun 2017)
Cellular Use Rating
In Model Organisms
(The reviewer did not leave any public comments)
by SERP
(on 29 Aug 2017)
Cellular Use Rating
In Model Organisms
(The reviewer did not leave any public comments)
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