Off target protein and potency: EHMT1 38 uM
Potency assay (off target):
MS-based histone methyltransferase assay. No activity was detected up to 45 uM BIX-01294 against PRMT1, SET7/9, ESET, SUV39H1,or H320R SUV39H1.
In DELFIA assay, no activity was detected to 10 uM against PRMT1 or SUV39H1.
Selectivity against non-family targets: